9
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Lab: Identifying Nutrients

Question 9 • HVA 26-27 IM Biology A

Check all the ways listed below that ensure accurate reading of volume from a graduated pipette.

Answer
A
tapping the base of the pipette on the table
B
using subtraction to determine the volume dispensed
C
making sure that you read the volume with the liquid inside the pipette at eye level
D
reading only the final volume of liquid after dispensing
E
ensuring that air bubbles are not in the column of liquid inside the pipette

Explanation

The volume dispensed from a graduated pipette is found by subtracting the final reading from the initial reading, so B is correct. Read the liquid level at eye height to avoid parallax error, and make sure there are no air bubbles in the liquid column, so C and E are correct. Tapping the pipette on a table is not a reliable way to improve the reading, and the final reading alone does not show how much was dispensed.

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