Answers26-27 Biology ALab: Building Proteins from RNA

Lab: Building Proteins from RNA Answers

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Answers:
direction,:3' to 5'
direction.:5' to 3'
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Answers:
base "words" that code for specific amino acids. They are:3
and never:nonoverlapping
the words.:have gaps between
4

overlapping✔ nonoverlapping and never

A
overlapping
B
✔ nonoverlapping
5

✔ have gaps betweenrepeat the words.

A
✔ have gaps between
B
repeat
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You transcribed DNA to make

A
tRNA
B
mRNA
C
DNA
D
protein
8

Check all ways below to ensure that the bases in RNA are properly paired.

A
The template DNA would be 3’ GUCUACAUGCCUGCUAUU 5’.
B
The mRNA strand is complementary to the DNA template strand.
C
During transcription, G in DNA pairs with C in mRNA, and A in DNA pairs with U in mRNA.
10

Number the steps in the correct order (1 through 5). Transcribe the DNA to create the mRNA. Match the mRNA codons to their tRNA anticodons and the attached amino acids. Perform dehydration synthesis to build the amino acid chain in the ribosome. Locate the start, stop, and other codons on the mRNA. Identify the antisense strand of the DNA within the cell nucleus.

Answers:
Number the steps in the correct order (1 through 5).:2
Number the steps in the correct order (1 through 5).:4
Number the steps in the correct order (1 through 5).:5
Number the steps in the correct order (1 through 5).:3
Number the steps in the correct order (1 through 5).:1
11

Suppose the following amino acid chain was created based on a student's transcription and translation:Tyr-Leu-Pro-MetIn the chain, which data show a possible source of error? Explain the error.

Answer:

The position of Met seems incorrect because methionine is the amino acid coded by the AUG start codon and should begin the chain. The student may have worked backward during transcription or translation.

12

How could you be sure you have located a section of DNA that encodes for a protein?

A
Write down the sequence to see what amino acids might be linked together.
B
Allow the DNA to be transcribed to RNA and see what protein results.
C
Compare the faulty protein to the DNA.
15

Check all ways below to ensure that the bases in RNA are properly paired.

A
The template DNA would be 3’ GUCUACAUGCCUGCUAUU 5’.
B
The mRNA strand is complementary to the DNA template strand.
C
During transcription, G in DNA pairs with C in mRNA, and A in DNA pairs with U in mRNA.
18

Suppose the following amino acid chain was created based on a student's transcription and translation:Tyr-Leu-Pro-MetIn the chain, which data show a possible source of error? Explain the error.

Answer:

The position of Met seems incorrect. The amino acid sequences of all proteins begin with Met because it is the amino acid that is attached to the anticodon for the AUG start codon. It looks like the student might have worked in a backward direction for transcription.

19

How could you be sure you have located a section of DNA that encodes for a protein?

A
Write down the sequence to see what amino acids might be linked together.
B
Allow the DNA to be transcribed to RNA and see what protein results.
C
Compare the faulty protein to the DNA.

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